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Creating a world without cancer.

Research

Resources: DNA Sequencing

DNA Sequencing

Overview

The DNA Sequencing Shared Resource (SEQ-SR) is part of the campus-wide Purdue University Genomics Facility that provides equal access to all Purdue University Center for Cancer Research (PCCR) investigators. Established in 1993, the SEQ-SR has provided PCCR members for the past 20 years with cost effective, quality services. The services provided by the DNA Sequencing Shared Resource (SEQ-SR) supported 40 Purdue Center for Cancer Research (PCCR) investigators’ research projects from all four of PCCR’s Research Programs in this current year. In addition to the standard DNA sample "Sanger" sequencing, the SEQ-SR is developing and refining next-generation services that are critical to the research needs of PCCR members’ future research plans. Members rely on the SEQ-SR to sequence genes and various gene constructs, and verify sequences containing ‘installed’ mutations, among other uses. The low-throughput laboratory service offered by the SEQ-SR facility additionally supports the Transgenic Mouse Core Facility Shared Resource (TMCF-SR). Specifically, the SEQ-SR helps ensure that the targeting and other DNA containing constructs that will be injected into rodents, are correct. Given the fairly low dollar cost of obtaining sequence data of a few samples, it is important to assess all the costs involved to researchers in utilizing a service of this type by convenience, rapid turn-around times, persistent data storage and availability of staff for consultation with the investigator.

Equipment

  • Illumina HiSeq 2500

  • 2x Illumina MiSeqs

  • ABI 3730XL (Sanger chemistry)

  • Agilent Bioanalyzer

  • Single cell Covaris for fragmenting DNA

Services & Pricing

Service PCCR Non-PCCR
Sanger Sequencing
(per sample)
$ 5.60 $ 9.20

For Mi-Seq and Hi-Seq, please contact the staff for further information.

Contact Information & Location


      • Shared Resource Operator/Bioinfoirmatics Specialist:
        Rick Westerman
      • E-mail: westerm@purdue.edu
      • Phone: (765) 494 - 0505
      • Building/Room: WSLR S049
  • Location of DNA Sequencing SR Facility

  • Purdue University Center for Cancer Research
    Hansen Life Sciences Research Building
    201 S. University Street
    West Lafayette, IN 47907-2064

  • Phone: (765) 494-9129
    Fax: (765) 494-9193
    Contact us with questions at:
    cancerresearch@purdue.edu

© Copyright 2014 The Purdue University Center for Cancer Research is the first NCI funded Center in Indiana.