Together, they focus on a shared goal:

Creating a world without cancer.

Research

Resources:
FLOW CYTOMETRY AND CELL SEPARATION

Flow Cytometry

Overview

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cellcycle distribution, apoptosis measurements, cytokine detection, data analysis and more. We will train those users interested in running their own samples on the BD Fortessa or the BC FC500. Facility staff will run all experiments on the FACS Aria III. The facility provides expert consultation for protocol development so that experiments are appropriately designed and implemented.

 

Capabilities Applications
  • Advanced flow cytometry acquisition and analysis
  • High speed, temperature controlled, sorting and analysis of live and rare cell populations
  • Single cell genomic gene analysis
  • Instrument training and data analysis
  • Data archiving and offline retrieval
  • FCS Express, FlowJo, and ModFitLt software packages
  • Expert consultation for assay or protocol development, grant proposals, and manuscripts
  • DNA cell cycle analysis
  • Apoptosis assays
  • Immunophenotyping
  • Intracellular phosphoprotein analysis
  • Antibody binding evaluation
  • Drug Response assays
  • Rare cell sorting and analysis

 

Equipment

BD Fortessa Cell Analyzer:

The BD Fortessa LSR flow cytometry cell analyzer has the same 5-laser setup as the ARIA cell sorter, with 18-color, 20-parameter detection and a high throughput microwell sample uptake system. This cytometer is available for operation by students and staff. This instrument is used for immunophenotyping, phosphoprotein analysis, DNA analysis, cell cycle and functional assays.

Fluidigm C1 System:

Fluidigm's C1 system is designed to isolate, process, and prepare individual cells for genome and transcriptome analysis. The instrument is used to sort cells in liquid suspension into 96 separate chambers to prepare them for DNA or RNA extraction. In partnership with the Purdue Genomics Core Facility, researchers apply NGS to identify changes in the genome or the gene expression patterns that affect cellular events and metabolic processes..

BD FACSAria III Cell Sorter:

The BD FACSAria III is a 5-laser, 14-color, 16-parameter high speed cell sorter with temperature control and housed within a biosafety cabinet for sorting of biohazardous samples. Wavelength excitation choices now include 405-nm, 488-nm, 561-nm, 633-nm and 355-nm (UV) lasers. With the advanced gel-coupled cuvette and octagon- and trigon-shaped detector arrays that maximize signal detection, the BD FACSAria III represents the state of the art in cell sorting. In addition, we will also do analysis-only experiments on the FACS Aria that require greater than four colors.

Beckman Coulter FC500:

The FC500 is a flow cytometry analyzer with two laser excitation wavelengths, 488nm and 615nm that can measure up to 5 colors simultaneously. This cytometer is available for operation by students and staff. It runs samples in 96 well plates or 12x75 tubes. This instrument is used for immunophenotyping, phophoprotein analysis, DNA analysis, cell cycle, and functional assays

 

Services & Pricing

Service PCCR Non-PCCR
BD Fortessa Cell Analyzer $ 32 $ 42
Fluidigm C1 System $ 355 $ 477
BD FACS Aria III Cell Sorter $ 30 $ 45
Beckman Coulter FC500 Analyzer $ 35 $ 42
Hourly Staff Rate $ 38 $ 57

Contact Information & Location

    • Jill Hutcroft
      • Shared Resource Manager: TBD
      • E-mail:
  • Location of Flow Cytometry SR Facility

  • Purdue University Center for Cancer Research
    Hansen Life Sciences Research Building
    201 S. University Street
    West Lafayette, IN 47907-2064

  • Phone: (765) 494-9129
    Fax: (765) 494-9193
    Contact us with questions at:
    cancerresearch@purdue.edu

© Copyright 2014 The Purdue University Center for Cancer Research is the first NCI funded Center in Indiana.