Together, they focus on a shared goal:

Creating a world without cancer.

Research

Resources: MACROMOLECULAR CRYSTALLOGRAPHY

Macromolecular Crystallography

Overview

The ability to determine the structure of proteins using X-ray crystallography has been one of the great scientific achievements over the past 50 to 60 years. It has provided broad insights into many areas of biology, ranging from the evolution of life, to understanding the chemistry of enzyme catalysis, to the control of cell growth and death. More recent applications have included producing the binding maps against which targeted drugs can be designed and evaluated leading to unparalleled growth in effective, safe therapeutics. As such, X-ray crystallography is an essential tool for the Purdue University Center for Cancer Research (PCCR) to carry out its mission - to uncover the fundamental sources of cell growth anomalies associated with cancer and to develop new and effective therapeutics through structure-based drug design.

Accordingly, the primary mission of the Macromolecular Crystallography Shared Resource (MM-SR) is to provide access to state-of-the-art technologies, hardware and expertise to PCCR members who use X-ray crystallography to address cancer-related questions and develop anti-cancer therapeutics. Formally established as a Shared Resource in 1998, this Resource has been committed to the support of macromolecular crystallography projects of PCCR researchers. The MM-SR has been a crucial resource to PCCR members studying cancer mechanisms and mutations, visualizing cancer-related drug targets and performing iterative, structure-based design of anti-cancer therapeutics. To enable the research of X-ray crystallography expert and non-expert PCCR members, the MM-SR provides and maintains in-house equipment and computational resources for data collection and processing, robotic systems for high-throughput micro crystallization experiments and for their imaging and storage, and temperature-controlled rooms.

The MM-SR’s services and support are critical to the PCCR members’ research needs because X-ray crystallography requires that sophisticated and dedicated resources be immediately and locally available to researchers. Because of the high associated costs and need for technical support, the X-ray data collection and crystallization hardware resources provided by the MM-SR are beyond the budgets and group size of most individual labs. This is especially true of the robotic crystallization and robotic visualization systems, which are key technologies that substantially accelerate the empirical process of identifying crystallization conditions and also allow, through microliter to nanoliter experiments, the preservation of precious biological material. By providing centralized hardware and expertise in a shared resource, MM-SR makes these essential technologies widely available to PCCR members, promoting productivity and scientific interaction.

Equipment

      • TPP "Mosquito" crystallization robot

      • Rigaku Desktop Minstrel crystal hotel and visualization system
      • TPP Dragonfly liquid handling robot
      • Three temperature controlled crystal growth rooms with associated microscopes.
      • Five Zeiss crystal microscopes, three with computer support for image capture.
      • Formulatrix visulation system with UV capability.

 

For x-ray diffraction:

Two Rigaku RU-200 x-ray generators with Oxford cryosystems in an inverted phi arrangement.

      • First with MSC Hires2 multilayer optics and an R-axis-IV++ image plate system optimized for use with a long unit cell axis that is characteristic of virus or membrane protein crystals.
      • Second with MSC Blue optics and an R-axis IV++ detector optimized for high flux to evaluate weakly diffracting protein crystals.

Services & Pricing

  PCCR Non-PCCR
X-ray Diffraction (per day) $ 45   $ 133  
Siemens 1000A Machine $ 21   $ 21  

Minstrel DT Imaging Robot,
Crystallization Rooms,
4DX Systems Crystal Spec,
Cryo-Xe-Siter,
Zeiss crystal microscopes,
Formulatrix visualization system with UV capability

Available at No Charge

 

Contact Information & Location

Steussy

Shared Resource Director: Calvin Steussy, MD, PhD
E-mail: csteussy@purdue.edu
Phone: (765) 494-7249

Schmidt

Shared Resource Manager: TJ Schmidt
Phone: (765) 496-3131


Location of Macromolecular Crystallography SR Facility

HOCK 320

location
  • Purdue University Center for Cancer Research
    Hansen Life Sciences Research Building
    201 S. University Street
    West Lafayette, IN 47907-2064

  • Phone: (765) 494-9129
    Fax: (765) 494-9193
    Contact us with questions at:
    cancerresearch@purdue.edu

© Copyright 2014 The Purdue University Center for Cancer Research is the first NCI funded Center in Indiana.