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Research

Resources: MASS SPECTROMETRY

Mass Spectometry

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Overview

Mass spectrometry is an essential analytical technique for the identification and structure elucidation of the wide variety of compounds that are important to the Purdue University Center for Cancer Research (PCCR) members’ research projects. These projects include studies on the design and synthesis of therapeutic agents for cancer treatment, the creation of diagnostic agents, and the investigation of basic cellular processes involved in cancer cell biology. Of additional importance is the ability of mass spectrometry to sort out reaction products to facilitate increased yield and understand where a reaction might be going wrong when the expected product is not obtained.

As part of the Campus-wide Mass Spectrometry Center, the Mass Spectrometry Shared Resource (MS-SR) provides PCCR members access to all of the facility’s mass spectrometers. This includes support for both routine analyses and collaborative analytical mass spectrometric capabilities (including problem solving by mass spectrometry). Taking advantage of the MS-SR staff’s experience, in addition to routine mass spectrometry analyses, considerable effort has been made to make each of the ionization techniques a center of excellence for solving the difficult mass spectrometry research problems associated with PCCR research projects. The MS-SR currently has eight mass spectrometers available to PCCR members. This includes Electrospray Ionization (ESI), Matrix-Assisted Laser Desorption Ionization (MALDI) and Atmospheric Pressure Chemical Ionization (APCI) for the analysis of nonvolatile components, Electron Impact (EI) and Chemical Ionization (CI) for volatile components, and Inductively Coupled Argon Plasma Ionization (ICP) for multi-element analysis.

Equipment

Drug Discovery Building (DRUG B059)

Applied Biosystems Voyager DE Pro

  • For matrix-assisted laser desorption ionization (MALDI) time-of-flight (ToF) mass spectrometer.
  • Analysis of intact, nonvolatile compounds to m/z 100,000. 

 

Agilent 5975C GC/MS

  • This quadrupole mass spectrometer utilizes an electron impact (EI) and chemical ionization (CI) probe coupled with capillary gas chromatography (GC/MS).
  • Volatile components to m/z 1000.
  • Specifications

 

Agilent 6550 Q-ToF

  • High performance ESI quadrupole time-of-flight (Q-ToF) instrument for LC-MS/MS.
  • 25-50k resolution for 50-3200 m/z.
  • 10,000 m/z extended mass range.
  • Capable of low detection limit quantification, multiple reaction monitoring (MRM), and compound identification via accurate mass.
  • Specifications

 

Applied Biosystems/MDS Sciex 4800 MALDI ToF/ToF

  • MALDI instrument capable of tandem mass spectrometry for compound characterization and identification.

 

Wetherill Chemistry Building (WTHR 144)

Thermo Fisher ELEMENT2

  • Inductively coupled plasma ionization mass spectrometer (ICP-MS).
  • Double focusing magnetic sector instrument for multi-element analyses including trace metals.
  • Specifications

 

Agilent 6320 Ion Trap

  • For electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and liquid chromatography/tandem mass spectrometry (LC/MS/MS).
  • Capable of compound characterization via multi-dimensional mass spectrometry (MSn).
  • Nonvolatile components to m/z 50,000.

 

Hewlett Packard 5989 GC/MS

  • Utilizes EI and CI with sample introduction either by GC or direct insertion probe.
  • Volatile components to m/z 1000

 

Thermo Fisher LTQ Orbitrap Classic

  • ESI, APCI, and Laser Ablation Electrospray Ionization (LAESI) imaging ion sources.
  • Capable of MS/MS and MSn in ion trap mode as well as high resolution, accurate mass analysis in orbitrap mode.
  • 100,000 resolution and less than 5 ppm mass accuracy
  • Orbitrap Specifications
  • LAESI Specifications

Services & Pricing

  Faculty in Subsidizing Department No Department Subsidy
Service PCCR
member
Non-PCCR
member
PCCR
member
Non-PCCR
member
MALDI $ 15.06 $ 31.62 $ 31.35 $ 47.91
LAESI $ 15.06 $ 31.62 $ 31.35 $ 47.91
GC/MS $ 15.06 $ 31.62 $ 31.35 $ 47.91
ICP $ 7.53 $ 15.81 $ 15.68 $ 23.96
PROBE EI/CI $ 15.06 $ 31.62 $ 31.35 $ 47.91
APCI $ 15.06 $ 31.62 $ 31.35 $ 47.91
ESPRAY  $ 15.06 $ 31.62 $ 31.35 $ 47.91
LC/MS $ 30.12 $ 63.24 $ 62.70 $ 95.82

*Prices are estimates representative of typical or average charges.

Per Sample Cost billed in 0.50 Hr Units.

Contact Information & Location

Ryan T. Hilger, PhD
Interim Director, Campus Wide Mass Spectrometry Center
rhilger1@purdue.edu

(765) 494-5234

 

Arlene Rothwell

Chemistry - WTHR Rooms 145/151
arothwell@purdue.edu
(765) 494-5469

 

Connie Bonham

Drug Discovery - DRUG Room B059
bonhamc@purdue.edu
(765) 494-1569 (office)
(765) 494-0707 (lab)

  • Purdue University Center for Cancer Research
    Hansen Life Sciences Research Building
    201 S. University Street
    West Lafayette, IN 47907-2064

  • Phone: (765) 494-9129
    Fax: (765) 494-9193
    Contact us with questions at:
    cancerresearch@purdue.edu

© Copyright 2014 The Purdue University Center for Cancer Research is the first NCI funded Center in Indiana.