Together, they focus on a shared goal:

Creating a world without cancer.

Research

Resources: TRANSGENIC MOUSE

Transgenic Mouse

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Overview

The ability to introduce foreign genes into the mammalian germ line or to selectively ablate endogenous genes from the mouse or rat genome, has proven to be one of the most powerful experimental tools available for understanding specific genetic requirements for tumor promoting regulatory pathways. In the area of oncology, transgenic mouse models have revealed the molecular pathways by which proto-oncogenes predispose cells to develop malignant tumors or how tumor suppressor genes maintain normal growth control. Transgenic strategies have also revolutionized the way we approach the complex problems associated with carcinogenesis, including the development of novel therapeutic intervention strategies. To support cancer research and utilization of this powerful technology, the Purdue University Center for Cancer Research (PCCR) Transgenic Mouse Core Facility Shared Resource (TMCF-SR) was established in 1998 through the generous support of the NCI and from funds obtained from the Purdue Office of the Vice President for Research.

The PCCR’s TMCF Shared Resource is a state-of-the-art facility that offers a large number of services to the Center membership, including the creation of transgenic and knock-out mouse and rat models for gain-of-function and loss-of-function experimental approaches to dissect transcriptional, signaling, and environmental influences on tumor initiation, progression and metastasis. Model systems based on transgenic and knock-in/knock-out strategies provide approaches that are developmentally, anatomically and physiologically relevant to human disease, and that can supplement traditional xenograft models for testing new anti-cancer therapies. Transgenic animal models provide valuable reagents for studying aspects of cancer cell biology, ranging from the regulatory mechanisms governing gene expression patterns to cell-cell interactions, cell cycle control and regulation of signal transduction pathways. Additionally, these models have been instrumental in dissecting the importance of immune cells and stromal infiltrates to tumor progression, and have helped to identify novel cancer stem cell lineages that are often resistant to conventional chemotherapeutics.

We offer genetic mutations in mice and rats by traditional methods such as pronuclear injection of plasmid DNA and gene targeting in ES cells, as well as newer technologies utilizing TALENs and CRISPRs.

TMCF Policies

Equipment

Services & Pricing

TGEF -Transgenic and Genome Editing Facility Rates

MOUSE

Service

PCCR Member Rate

Internal Rate

External Rate

Genome Editing

Transgenic Mouse Production

$2,575

$6,230

$9,657

Additional TG Injection Session

$715

$1,375

$2,131

CRISPR KI mice

$2,760

$7,700

$11,935

CRISPR KO mice

$2,095

$5,170

$8,014

CRISPR Validation

$60

$260

$403

Additional CRISPR Injection Session

$750

$1,725

$2,674

Gene Targeting + Blast Injection

$7,500

$18,950

$29,373

Blast Injection of ES Cells

$4,900

$11,660

$18,073

Assisted Reproduction

Rederivation by Embryo Transfer

$235

$410

$636

Embryo Cryopreservation

$470

$1,375

$2,131

Speed (Embryo) Cryopreservation

$885

$1,740

$2,697

Sperm Cryopreservation

$130

$270

$419

In Vitro Fertilization

$335

$900

$1,395

Speed Expansion

$750

$1,840

$2,852

Cryo Bank per Line charge

$18

$22

$34

Tissue Culture

Chromosome Counting

$100

$360

$558

Mouse Embryonic Fibroblast Production

$450

$970

$1,504

Molecular Biology

PCR Design + Optimization

$175

$175

$271

PCR Optimization

$115

$115

$178

Genomic DNA Isolation and Genotyping

$10

$10

$16

Husbandry

Production Colony

$14

$14

N/A

Mouse Colony Set Up

$100

$130

N/A

Animal Shipment (Domestic) + Packaging at cost

$140

$140

$217

Animal Shipment (International) + Packaging at cost

$235

$235

$364

Miscellaneous

Animal Labor Rate

$47

$47

$73

Lab Labor Rate

$38

$38

$59

Consultation Rate

$80

$80

$124

 

TGEF -Transgenic and Genome Editing Facility Rates

RAT

Service

PCCR Member Rate

Internal Rate

External Rate

Genome Editing

Rat Transgenic Production

$3,810

$8,410

$13,036

Additional TG Injection Session

$900

$2,010

$3,116

CRISPR KI Rats

$4,000

$9,580

$14,849

CRISPR KO Rats

$2,600

$6,040

$9,362

CRISPR Validation

$75

$280

$434

Additional CRISPR Injection Session

$1,000

$2,170

$3,364

Assisted Reproduction

Rederivation by Embryo Transfer

$420

$610

$946

Embryo Cryopreservation

$990

$1,900

$2,945

Cryo Bank per Line charge

$18

$22

$34

Molecular Biology

PCR Design + Optimization

$175

$175

$271

PCR Optimization

$115

$115

$178

Genomic DNA Isolation and Genotyping

$10

$10

$16

Husbandry

Animal Shipment (Domestic) + Packaging at cost

$140

$140

$217

Animal Shipment (International) + Packaging at cost

$235

$235

$364

Miscellaneous

Animal Labor Rate

$47

$47

$73

Lab Labor Rate

$38

$38

$59

Consultation Rate

$80

$80

$124

 

 

Contact Information & Location

  • Purdue University Center for Cancer Research
    Hansen Life Sciences Research Building
    201 S. University Street
    West Lafayette, IN 47907-2064

  • Phone: (765) 494-9129
    Fax: (765) 494-9193
    Contact us with questions at:
    cancerresearch@purdue.edu

© Copyright 2014 The Purdue University Center for Cancer Research is the first NCI funded Center in Indiana.